Author: Lee, Charlie Wah Heng; Koh, Chee Wee; Chan, Yang Sun; Aw, Pauline Poh Kim; Loh, Kuan Hon; Han, Bing Ling; Thien, Pei Ling; Nai, Geraldine Yi Wen; Hibberd, Martin L.; Wong, Christopher W.; Sung, Wing-Kin
Title: Large-scale evolutionary surveillance of the 2009 H1N1 influenza A virus using resequencing arrays Document date: 2010_2_25
ID: 1rhy8td0_8
Snippet: Viral RNA from the diagnostic swabs or RNA extracted from MDCK cell cultures was extracted using the DNA minikit (Qiagen, Inc, Valencia, CA, USA) according to manufacturer's instructions. RNA was reverse-transcribed to cDNA using customized random primers designed using LOMA (11) and then amplified by PCR using proprietary H1N1(2009) specific primers. The presence of H1N1(2009) in the samples was confirmed using a separate real-time PCR assay bas.....
Document: Viral RNA from the diagnostic swabs or RNA extracted from MDCK cell cultures was extracted using the DNA minikit (Qiagen, Inc, Valencia, CA, USA) according to manufacturer's instructions. RNA was reverse-transcribed to cDNA using customized random primers designed using LOMA (11) and then amplified by PCR using proprietary H1N1(2009) specific primers. The presence of H1N1(2009) in the samples was confirmed using a separate real-time PCR assay based on the published primer sequences from the Centre for Disease Control and Prevention (CDC), USA.
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