Author: Kim, Nam-Gun; Kim, Myeong-Ae; Park, Young-Il; Jung, Tae-Sung; Son, Seong-Wan; So, ByungJae; Kang, Hwan-Goo
Title: Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin Document date: 2015_12_17
ID: 5i84nomn_16
Snippet: The presence and specificity of antibodies were tested by indirect, competitive ELISA as described below. The microplates were coated with ENR-BSA dissolved in 0.5 M carbonate buffer (100 ng per well, 100 ïL). Following overnight incubation at 4 o C, plates were washed three times with washing buffer and blocked with 1% casein in PBS (w/v, 250 ïL/well), after which they were incubated for 1 h at 37 o C. The blocking solution was then removed,.....
Document: The presence and specificity of antibodies were tested by indirect, competitive ELISA as described below. The microplates were coated with ENR-BSA dissolved in 0.5 M carbonate buffer (100 ng per well, 100 ïL). Following overnight incubation at 4 o C, plates were washed three times with washing buffer and blocked with 1% casein in PBS (w/v, 250 ïL/well), after which they were incubated for 1 h at 37 o C. The blocking solution was then removed, after which PBS buffer or competitor in PBS buffer (80 ïL/well) was added to each well, followed by the addition of serum or culture supernatants(80 ïL/well). Samples were then incubated at room temperature for 1 h, after which they were washed three times, and 100 ïL of anti-mouse IgG-HRP conjugate (dilution 1 : 1000 in 1% skim milk) was added and incubated for 1 h. Each well was washed with washing buffer and 100 ïL of TMB solution for 5 min, after which color development was halted by adding 100 ïL of 2N H2SO4, and the absorbance at 450 nm was measured using a microtiter plate reader.
Search related documents:
Co phrase search for related documents- overnight incubation and plate reader: 1, 2
- overnight incubation and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- overnight incubation and skim milk: 1
- overnight incubation and wash buffer: 1
- PBS buffer and plate reader: 1
- PBS buffer and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- PBS buffer and skim milk: 1, 2, 3, 4
- PBS buffer and TMB solution: 1, 2, 3
- PBS buffer and wash buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- plate reader and room temperature: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- plate reader and TMB solution: 1, 2, 3, 4
- plate reader and wash buffer: 1
- room temperature and skim milk: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
- room temperature and specificity presence: 1
- room temperature and TMB solution: 1, 2, 3, 4
- room temperature and wash buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- room temperature incubate and skim milk: 1
- time wash and wash buffer: 1
- TMB solution and wash buffer: 1
Co phrase search for related documents, hyperlinks ordered by date