Title: Membrane insertion of gap junction connexins: polytopic channel forming membrane proteins Document date: 1994_10_2
ID: 1gqffey0_6
Snippet: Rough microsomal membranes used in this study were either purchased from Promege Biotech (Madison, WI) or were prepared as described below. Salt-extracted microsomal membranes, purified signal recognition particle (SRP), and plasmid pBP4 encoding bovine preprolactin were kindly provided by Dr. D. Zopf and Dr. P. Walter (University of California, San Francisco, CA). Plasmid pCh2934-SP encoding the chicken AChR ct7 subunit was kindly provided by Dr.....
Document: Rough microsomal membranes used in this study were either purchased from Promege Biotech (Madison, WI) or were prepared as described below. Salt-extracted microsomal membranes, purified signal recognition particle (SRP), and plasmid pBP4 encoding bovine preprolactin were kindly provided by Dr. D. Zopf and Dr. P. Walter (University of California, San Francisco, CA). Plasmid pCh2934-SP encoding the chicken AChR ct7 subunit was kindly provided by Dr. R. Sch6pfer (Center for Molecular Biology, Heidelberg, Germany). Antibodies/31 1-6, GAP 10, and anti bovine protein disulfide isomerase (PDI) were kindly provided by Dr. B. Risek (The Scripps Research Institute, La Jolla, CA), Dr. W. H. Evans (University of Wales, Heath Park, Cardiff, U. K.), and Dr. M. R. Jackson (The Scripps Research Institute, La Jolla, CA), respectively. A mouse hybridoma cell line producing a/5, GJ protein-specific monoclonal antibody (M12.13) was kindly provided by Dr. D. A. Goodenough (Harvard Medical School, Boston, MA) .
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