Author: Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao
Title: miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1 Document date: 2013_12_6
ID: 4vo7n6nh_23
Snippet: (h) Transcriptional activation from the predicted Smad3 binding sites was induced by TGF-b in RAW cells. Cells were co-transfected with luciferase reporter including Smad3-binding sites and pRL-TK. After 48 h, cells were treated with TGF-b for 4 h and luciferase values were measured. pRL-TK was co-transfected as an internal control to correct the differences in both transfection and harvest efficiencies. The firefly luciferase activity of each sa.....
Document: (h) Transcriptional activation from the predicted Smad3 binding sites was induced by TGF-b in RAW cells. Cells were co-transfected with luciferase reporter including Smad3-binding sites and pRL-TK. After 48 h, cells were treated with TGF-b for 4 h and luciferase values were measured. pRL-TK was co-transfected as an internal control to correct the differences in both transfection and harvest efficiencies. The firefly luciferase activity of each sample was normalized to the Renilla luciferase activity. Data are shown as mean±s.d. (n ¼ 4) . Similar results were obtained in three independent experiments. *P ¼ 0.0008, **P ¼ 0.0002, ***P ¼ 0.0006 (two-tailed Student's t-test).
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