miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1

Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao

In adult mice, HSCs are originally described as precursors to all different subpopulations of DCs in the bone marrow. Upon inflammatory stimulation or uptake of pathogenic antigens, immature DCs (imDCs) undergo maturation and differentiate into mature DCs (maDCs), which are originally considered to be terminally differentiated 14 . However, our previous study demonstrated that splenic stromal cells, which mimic the secondary lymph organ microenvironment, can drive maDCs to further differentiate into regulatory DCs (DCreg) 15 . Therefore, the process of DC development and differentiation is very interesting, as it includes one development process (HSCs-imDCs), one maturation process (imDCs-maDCs) and one differentiation process (maDCs-DCreg), thus providing a good cell model to investigate how miRNAs are involved in different processes of cell differentiation and functional regulation. Up to now, a few studies have investigated this topic [16] [17] [18] [19] ; however, a comprehensive profile of miRNAs in mouse bone marrowderived DCs at different differentiation stages remain largely unknown.