Author: Muhammad, Azharuddin; Toufeeq, Shahzad; Yu, Hai-Zhong; Wang, Jie; Zhang, Shang-Zhi; Li, Bing; Li, Zhen; Yang, Li-Ang; Hu, Pei; Ma, Yan; Xu, Jia-Ping
Title: Molecular Characterization of Two Mitogen-Activated Protein Kinases: p38 MAP Kinase and Ribosomal S6 Kinase From Bombyx mori (Lepidoptera: Bombycidae), and Insight Into Their Roles in Response to BmNPV Infection Document date: 2019_2_2
ID: 2s3x6sj8_35
Snippet: To evaluate iTRAQ database, we obtained the expression profile of Bmp38 and BmS6K in the midgut infected with BmNPV, and results were evaluated by RT-qPCR and western blotting. The RT-qPCR results of Bmp38 indicated that its expression was upregulated in infected group (P50+ and BC9+) than control group (P50− and BC9−). When compared between P50 (susceptible strain) and BC9 (resistant strain), we observed that Bmp38 expression was higher in B.....
Document: To evaluate iTRAQ database, we obtained the expression profile of Bmp38 and BmS6K in the midgut infected with BmNPV, and results were evaluated by RT-qPCR and western blotting. The RT-qPCR results of Bmp38 indicated that its expression was upregulated in infected group (P50+ and BC9+) than control group (P50− and BC9−). When compared between P50 (susceptible strain) and BC9 (resistant strain), we observed that Bmp38 expression was higher in BC9 more than P50 at 12, 24, 48, and 72 h (Fig. 7A ). In addition, western blot results showed that Bmp38 translation profile is correlated with Bmp38 transcription profile (Fig. 7B) .
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