Author: Gardner, Shea N.; Hiddessen, Amy L.; Williams, Peter L.; Hara, Christine; Wagner, Mark C.; Colston, Bill W.
Title: Multiplex primer prediction software for divergent targets Document date: 2009_9_16
ID: 7658dmvk_4
Snippet: Finally, we empirically multiplexed a set of 16 short (10-mer) primers designed for the Poxviridae family. This demonstrated specific amplification of the expected viral fragment from vaccinia Lister strain. This preliminary demonstration suggests that specific amplification with family-level multiplexed sets of short primers might be feasible for viral detection and discovery, particularly as a means for selective enrichment of viral target for .....
Document: Finally, we empirically multiplexed a set of 16 short (10-mer) primers designed for the Poxviridae family. This demonstrated specific amplification of the expected viral fragment from vaccinia Lister strain. This preliminary demonstration suggests that specific amplification with family-level multiplexed sets of short primers might be feasible for viral detection and discovery, particularly as a means for selective enrichment of viral target for downstream amplicon characterization (e.g. sequencing or probe hybridization).
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