Author: Yu, Xiaobo; Song, Lusheng; Petritis, Brianne; Bian, Xiaofang; Wang, Haoyu; Viloria, Jennifer; Park, Jin; Bui, Hoang; Li, Han; Wang, Jie; Liu, Lei; Yang, Liuhui; Duan, Hu; McMurray, David N.; Achkar, Jacqueline M.; Magee, Mitch; Qiu, Ji; LaBaer, Joshua
Title: Multiplexed Nucleic Acid Programmable Protein Arrays Document date: 2017_9_20
ID: 7t1o19kn_28
Snippet: The schematic illustration of how M-NAPPA arrays are processed is shown in Figure 1A . Using a standard pin-based arrayer, each spot on M-NAPPA contains plasmids encoding for different proteins-of-interest with the same fusion tag. The genes are then transcribed and translated into recombinant proteins in two hours using a cell-free expression system, and captured to the slide surface in situ via a fusion tag antibody......
Document: The schematic illustration of how M-NAPPA arrays are processed is shown in Figure 1A . Using a standard pin-based arrayer, each spot on M-NAPPA contains plasmids encoding for different proteins-of-interest with the same fusion tag. The genes are then transcribed and translated into recombinant proteins in two hours using a cell-free expression system, and captured to the slide surface in situ via a fusion tag antibody.
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