Author: Yu, Xiaobo; Song, Lusheng; Petritis, Brianne; Bian, Xiaofang; Wang, Haoyu; Viloria, Jennifer; Park, Jin; Bui, Hoang; Li, Han; Wang, Jie; Liu, Lei; Yang, Liuhui; Duan, Hu; McMurray, David N.; Achkar, Jacqueline M.; Magee, Mitch; Qiu, Ji; LaBaer, Joshua
Title: Multiplexed Nucleic Acid Programmable Protein Arrays Document date: 2017_9_20
ID: 7t1o19kn_42
Snippet: To test whether M-NAPPA can be used to detect proteomic serological response, we screened ten serum samples from patients with type 1 diabetes that had been previously characterized using NAPPA arrays [20] . A dozen hits were observed with M-NAPPA and NAPPA (Figure 5) . Forty-nine of the 53 antigens (92.5%) identified by NAPPA were also detected by M-NAPPA. Four antigens, however, were detected with only one platform (i.e., two with NAPPA, two wi.....
Document: To test whether M-NAPPA can be used to detect proteomic serological response, we screened ten serum samples from patients with type 1 diabetes that had been previously characterized using NAPPA arrays [20] . A dozen hits were observed with M-NAPPA and NAPPA (Figure 5) . Forty-nine of the 53 antigens (92.5%) identified by NAPPA were also detected by M-NAPPA. Four antigens, however, were detected with only one platform (i.e., two with NAPPA, two with M-NAPPA). These uncommon discrepancies may be due to variations in surface chemistry, plasmid concentration, printing or array processing.
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