Author: Yu, Xiaobo; Song, Lusheng; Petritis, Brianne; Bian, Xiaofang; Wang, Haoyu; Viloria, Jennifer; Park, Jin; Bui, Hoang; Li, Han; Wang, Jie; Liu, Lei; Yang, Liuhui; Duan, Hu; McMurray, David N.; Achkar, Jacqueline M.; Magee, Mitch; Qiu, Ji; LaBaer, Joshua
Title: Multiplexed Nucleic Acid Programmable Protein Arrays Document date: 2017_9_20
ID: 7t1o19kn_47
Snippet: Here, we developed a new strategy, M-NAPPA, that significantly increases the number proteins that can be tested per slide multiple-fold. By combining five different plasmids within one feature, >10k proteins can be printed on one microscope slide for HT, low cost analyses when compared to studies using one-plasmid-per-feature arrays. The multiplexed hits that are identified with M-NAPPA can then be deconvoluted during the subsequent verification .....
Document: Here, we developed a new strategy, M-NAPPA, that significantly increases the number proteins that can be tested per slide multiple-fold. By combining five different plasmids within one feature, >10k proteins can be printed on one microscope slide for HT, low cost analyses when compared to studies using one-plasmid-per-feature arrays. The multiplexed hits that are identified with M-NAPPA can then be deconvoluted during the subsequent verification step (Figure 8) .
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