Author: Daniels, Keith A.; Devora, Gene; Lai, Wayne C.; O'Donnell, Carey L.; Bennett, Michael; Welsh, Raymond M.
Title: Murine Cytomegalovirus Is Regulated by a Discrete Subset of Natural Killer Cells Reactive with Monoclonal Antibody to Ly49h Document date: 2001_7_2
ID: 6n3dmle6_27
Snippet: We questioned whether the IFN-â¥-producing NK cells elicited by these different viruses were also biased in their expression of 1F8. Experiment 1 in Table I compares IFN-⥠production in response to either of the two LCMV strains with MCMV, and even though the total IFN-⥠response was much lower, the LCMV-induced NK cells had Figure 5 . Lack of selective IFN-⥠production by Ly49D-or Ly49G2-expressing NK cells. Gated NK1.1 Ï© CD3 Ϫ NK cells.....
Document: We questioned whether the IFN-â¥-producing NK cells elicited by these different viruses were also biased in their expression of 1F8. Experiment 1 in Table I compares IFN-⥠production in response to either of the two LCMV strains with MCMV, and even though the total IFN-⥠response was much lower, the LCMV-induced NK cells had Figure 5 . Lack of selective IFN-⥠production by Ly49D-or Ly49G2-expressing NK cells. Gated NK1.1 Ï© CD3 Ϫ NK cells from the spleens and PECs of 2-d MCMV-infected C57BL/6 mice were stained with Abs to Ly49D, G2, C/I (mAb 5E6), or C/I/H (mAb 1F8) and tested for spontaneous IFN-⥠production. Isotype controls for the Ly49 mAb are displayed. Isotype controls for the IFN-⥠Ab were Ͻ1%, as seen in Fig. 4 . a similar if not greater skewing of the IFN-⥠response within the NK cell population defined by mAb 1F8, and less so in that defined by mAb 5E6. A second experiment (Table I, Exp. 2) with LCMV, MHV, and VV similarly showed a preponderance in IFN-⥠responsiveness within the cell populations defined by mAb 1F8; the peritoneal cell data from this experiment are depicted in Fig. 6 , which shows a predominant skewing of the IFN-⥠response to 1F8 Ï© cells, particularly for the LCMV and VV infections. These results suggest that the propensity of the 1F8 Ï© cells to proportionally dominate the IFN-⥠response of NK cells is profound but not specific to a given virus. It should also be noted that 5E6 Ï© cells, which may or may not express Ly49H, had relatively high expression of IFN-⥠in some of the samples (Table I) .
Search related documents:
Co phrase search for related documents- Abs stain and Ï© cell: 1
- Abs stain and isotype control: 1
- Abs stain and NK cell: 1
- cell population and different virus: 1, 2, 3, 4, 5, 6, 7, 8, 9
- cell population and Ï© cell: 1, 2, 3, 4, 5
- cell population and isotype control: 1, 2, 3
- cell population and NK cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- different virus and give virus: 1, 2, 3, 4, 5, 6, 7, 8, 9
- different virus and Ï© cell: 1
- different virus and NK cell: 1, 2, 3, 4, 5, 6
- different virus and NK cell response: 1
- Ï© cell and isotype control: 1, 2
- Ï© cell and LCMV induce: 1
- Ï© cell and LCMV strain: 1, 2
- Ï© cell and NK cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- Ï© cell and NK cell response: 1
- isotype control and NK cell: 1, 2, 3
- LCMV strain and NK cell: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date