Selected article for: "cell population and ifn response"

Author: Daniels, Keith A.; Devora, Gene; Lai, Wayne C.; O'Donnell, Carey L.; Bennett, Michael; Welsh, Raymond M.
Title: Murine Cytomegalovirus Is Regulated by a Discrete Subset of Natural Killer Cells Reactive with Monoclonal Antibody to Ly49h
  • Document date: 2001_7_2
  • ID: 6n3dmle6_27
    Snippet: We questioned whether the IFN-␥-producing NK cells elicited by these different viruses were also biased in their expression of 1F8. Experiment 1 in Table I compares IFN-␥ production in response to either of the two LCMV strains with MCMV, and even though the total IFN-␥ response was much lower, the LCMV-induced NK cells had Figure 5 . Lack of selective IFN-␥ production by Ly49D-or Ly49G2-expressing NK cells. Gated NK1.1 ϩ CD3 Ϫ NK cells.....
    Document: We questioned whether the IFN-␥-producing NK cells elicited by these different viruses were also biased in their expression of 1F8. Experiment 1 in Table I compares IFN-␥ production in response to either of the two LCMV strains with MCMV, and even though the total IFN-␥ response was much lower, the LCMV-induced NK cells had Figure 5 . Lack of selective IFN-␥ production by Ly49D-or Ly49G2-expressing NK cells. Gated NK1.1 ϩ CD3 Ϫ NK cells from the spleens and PECs of 2-d MCMV-infected C57BL/6 mice were stained with Abs to Ly49D, G2, C/I (mAb 5E6), or C/I/H (mAb 1F8) and tested for spontaneous IFN-␥ production. Isotype controls for the Ly49 mAb are displayed. Isotype controls for the IFN-␥ Ab were Ͻ1%, as seen in Fig. 4 . a similar if not greater skewing of the IFN-␥ response within the NK cell population defined by mAb 1F8, and less so in that defined by mAb 5E6. A second experiment (Table I, Exp. 2) with LCMV, MHV, and VV similarly showed a preponderance in IFN-␥ responsiveness within the cell populations defined by mAb 1F8; the peritoneal cell data from this experiment are depicted in Fig. 6 , which shows a predominant skewing of the IFN-␥ response to 1F8 ϩ cells, particularly for the LCMV and VV infections. These results suggest that the propensity of the 1F8 ϩ cells to proportionally dominate the IFN-␥ response of NK cells is profound but not specific to a given virus. It should also be noted that 5E6 ϩ cells, which may or may not express Ly49H, had relatively high expression of IFN-␥ in some of the samples (Table I) .

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