Author: Rao, Xiaoquan; Zhao, Shi; Braunstein, Zachary; Mao, Hong; Razavi, Michael; Duan, Lihua; Wei, Yingying; Toomey, Amelia C.; Rajagopalan, Sanjay; Zhong, Jixin
Title: Oxidized LDL upregulates macrophage DPP4 expression via TLR4/TRIF/CD36 pathways Document date: 2019_2_7
ID: 1n7xjjd5_7
Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood through density gradient centrifugation using Ficoll. In brief, blood collected into EDTA-coated tubes was diluted 1:1 with sterile PBS and layered on Ficoll-Paque Plus (GE Healthcare, Piscataway, NJ), followed by centrifugation for 30 min at 500g without applying a brake. The PBMCs in the interface were carefully removed and washed twice with PBS. PBMCs were the.....
Document: Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood through density gradient centrifugation using Ficoll. In brief, blood collected into EDTA-coated tubes was diluted 1:1 with sterile PBS and layered on Ficoll-Paque Plus (GE Healthcare, Piscataway, NJ), followed by centrifugation for 30 min at 500g without applying a brake. The PBMCs in the interface were carefully removed and washed twice with PBS. PBMCs were then placed in a 6-well plate for 2 h, and then adherent cells (monocytes) were cultured in RPMI-1640 medium supplemented with 10% FBS and 10 ng/mL recombinant human macrophage colony-stimulating factor (R&D, Minneapolis, MN) for 4 days. Media were replaced once at day 2.
Search related documents:
Co phrase search for related documents- Ficoll gradient centrifugation and human peripheral blood: 1
- Ficoll gradient centrifugation and peripheral blood: 1, 2, 3, 4, 5, 6
- gradient centrifugation and human peripheral blood: 1
- gradient centrifugation and peripheral blood: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- gradient centrifugation and peripheral blood mononuclear: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- human macrophage and peripheral blood: 1, 2, 3, 4, 5
- human macrophage and peripheral blood mononuclear: 1, 2, 3, 4
- human macrophage and peripheral blood mononuclear cell: 1
- human peripheral blood and PBMCs peripheral blood mononuclear cell: 1, 2, 3, 4, 5
- human peripheral blood and peripheral blood: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- human peripheral blood and peripheral blood mononuclear: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- human peripheral blood and peripheral blood mononuclear cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
- MN Minneapolis and peripheral blood: 1
- PBMCs peripheral blood mononuclear cell and peripheral blood: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34
- PBMCs peripheral blood mononuclear cell and peripheral blood mononuclear: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34
- PBMCs peripheral blood mononuclear cell and peripheral blood mononuclear cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33
- PBS twice wash and twice wash: 1, 2, 3
- peripheral blood and twice wash: 1
- peripheral blood mononuclear and twice wash: 1
Co phrase search for related documents, hyperlinks ordered by date