Selected article for: "density gradient centrifugation and peripheral blood mononuclear"
Author: Rao, Xiaoquan; Zhao, Shi; Braunstein, Zachary; Mao, Hong; Razavi, Michael; Duan, Lihua; Wei, Yingying; Toomey, Amelia C.; Rajagopalan, Sanjay; Zhong, Jixin
Title: Oxidized LDL upregulates macrophage DPP4 expression via TLR4/TRIF/CD36 pathways
  • Document date: 2019_2_7
    • ID: 1n7xjjd5_7
    Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood through density gradient centrifugation using Ficoll. In brief, blood collected into EDTA-coated tubes was diluted 1:1 with sterile PBS and layered on Ficoll-Paque Plus (GE Healthcare, Piscataway, NJ), followed by centrifugation for 30 min at 500g without applying a brake. The PBMCs in the interface were carefully removed and washed twice with PBS. PBMCs were the.....
    Document: Peripheral blood mononuclear cells (PBMCs) were isolated from human peripheral blood through density gradient centrifugation using Ficoll. In brief, blood collected into EDTA-coated tubes was diluted 1:1 with sterile PBS and layered on Ficoll-Paque Plus (GE Healthcare, Piscataway, NJ), followed by centrifugation for 30 min at 500g without applying a brake. The PBMCs in the interface were carefully removed and washed twice with PBS. PBMCs were then placed in a 6-well plate for 2 h, and then adherent cells (monocytes) were cultured in RPMI-1640 medium supplemented with 10% FBS and 10 ng/mL recombinant human macrophage colony-stimulating factor (R&D, Minneapolis, MN) for 4 days. Media were replaced once at day 2.

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