Selected article for: "molecular assay and pan primer"
Author: Cordey, Samuel; Thomas, Yves; Suter, Patricia; Kaiser, Laurent
Title: Pilot Evaluation of RT-PCR/Electrospray Ionization Mass Spectrometry (PLEX-ID/Flu assay) on Influenza-Positive Specimens
  • Document date: 2012_5_9
    • ID: 085v7n6k_4
    Snippet: A first version of the influenza assay (Abbott Molecular, Des Plaines, IL, USA) performed on the T5000 instrument (Ibis/Abbott, Carlsbad, CA, USA) was validated for influenza A (including A(H3N2) and H5N1) and B isolates collected between 1999 and 2006 [13] . Sensitivity and specificity were estimated to reach 97% and 98%, respectively. ESI-MS analysis was also capable of identifying viral reassortments or co-infections (i.e., a mixed population).....
    Document: A first version of the influenza assay (Abbott Molecular, Des Plaines, IL, USA) performed on the T5000 instrument (Ibis/Abbott, Carlsbad, CA, USA) was validated for influenza A (including A(H3N2) and H5N1) and B isolates collected between 1999 and 2006 [13] . Sensitivity and specificity were estimated to reach 97% and 98%, respectively. ESI-MS analysis was also capable of identifying viral reassortments or co-infections (i.e., a mixed population). More recently, this assay reported a sensitivity and specificity of 94.1% and 97%, respectively, for A(H1N1)pdm detection [11] . Since then, the assay has been redesigned and updated. This "PLEX-ID/Flu assay" includes one pan-influenza primer set targeting the PB1 segment, five pan-influenza-A primer pairs targeting individually NP, M1, PA, PB2 and NS1 genes, one pan-influenza B primer pair targeting the PB2 segment, and two additional primer pairs targeting two surface antigens HA (H1) and NA (N1) genes [12] .

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