Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity Document date: 1998_6_15
ID: 78fjem8s_5
Snippet: Mutant HAs were generated using the method of Kunkel et al. (1987) on wild-type (wt)-HA (X:31 strain) cDNA in the plasmid pSM. Mutant HA cDNAs were sequenced to confirm that the desired mutations had been introduced, but that second site mutations had not. Mutant HAs were subcloned into the vector pCB6 for expression in 293T cells......
Document: Mutant HAs were generated using the method of Kunkel et al. (1987) on wild-type (wt)-HA (X:31 strain) cDNA in the plasmid pSM. Mutant HA cDNAs were sequenced to confirm that the desired mutations had been introduced, but that second site mutations had not. Mutant HAs were subcloned into the vector pCB6 for expression in 293T cells.
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