Author: Martin, Baptiste; Coutard, Bruno; Guez, Théo; Paesen, Guido C; Canard, Bruno; Debart, Françoise; Vasseur, Jean-Jacques; Grimes, Jonathan M; Decroly, Etienne
Title: The methyltransferase domain of the Sudan ebolavirus L protein specifically targets internal adenosines of RNA substrates, in addition to the cap structure Document date: 2018_9_6
ID: 243u68j8_10
Snippet: The coding sequence of the human RNA N7 MTase (RNMT), hMPV MTase, Zika virus (ZIKV) and dengue virus (DV) MTases were cloned in fusion with the coding sequence of a hexa-histidine tag, and corresponding recombinant proteins were expressed and purified as previously described (26) (27) (28) (29) . The vaccinia virus VP39 MTase was purchased (New England Biolabs)......
Document: The coding sequence of the human RNA N7 MTase (RNMT), hMPV MTase, Zika virus (ZIKV) and dengue virus (DV) MTases were cloned in fusion with the coding sequence of a hexa-histidine tag, and corresponding recombinant proteins were expressed and purified as previously described (26) (27) (28) (29) . The vaccinia virus VP39 MTase was purchased (New England Biolabs).
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