Selected article for: "detection kit and PCR multiplex"

Author: Chen, Jing; Li, Xiaoguang; Wang, Wei; Jia, Ying; Lin, Fei; Xu, Jie
Title: The prevalence of respiratory pathogens in adults with community-acquired pneumonia in an outpatient cohort
  • Document date: 2019_7_30
  • ID: 6ghxcphh_8
    Snippet: All analyses were performed with Statistical 19.0 and Microsoft Excel 2007. General data are presented as a percentage (P) or mean ± SD. Differences in categorical variables between groups were compared using the χ2 test. A single-tailed P-value of <0.05 was considered statistically significant. . The housekeeping gene RNaseP was used as the internal standard in the multiplex real-time RT-PCR assay. There were positive and negative controls in .....
    Document: All analyses were performed with Statistical 19.0 and Microsoft Excel 2007. General data are presented as a percentage (P) or mean ± SD. Differences in categorical variables between groups were compared using the χ2 test. A single-tailed P-value of <0.05 was considered statistically significant. . The housekeeping gene RNaseP was used as the internal standard in the multiplex real-time RT-PCR assay. There were positive and negative controls in the RT-PCR Taq kit. In the detection kit for the respiratory viruses, positive controls were the artificially synthesized virus RNA with target sequences, and negative controls were RNasefree and DNase-free water. In the detection kit for the respiratory bacteria, positive controls were the bacterial pathogens and RNaseP of target sequence plasmids, and negative controls were RNase-free and DNase-free water.

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