Author: Kim, Sung-Kwon; Cornberg, Markus; Wang, Xiaoting Z.; Chen, Hong D.; Selin, Liisa K.; Welsh, Raymond M.
Title: Private specificities of CD8 T cell responses control patterns of heterologous immunity Document date: 2005_2_21
ID: 55gi6gyx_43
Snippet: Viruses. LCMV, strain Armstrong, and its highly disseminating variant, clone 13, are ambisense RNA viruses in the arenavirus family and were propagated in BHK21 baby hamster kidney cells (32, 33) . VV, strain WR, a DNA virus in the poxvirus family, was propagated in NCTC 929 cells (32) . PV, strain AN3739, a New World arenavirus only distantly related to LCMV, was propagated in BHK21 cells (33) . CFSE labeling. Leukocytes from donor mice were har.....
Document: Viruses. LCMV, strain Armstrong, and its highly disseminating variant, clone 13, are ambisense RNA viruses in the arenavirus family and were propagated in BHK21 baby hamster kidney cells (32, 33) . VV, strain WR, a DNA virus in the poxvirus family, was propagated in NCTC 929 cells (32) . PV, strain AN3739, a New World arenavirus only distantly related to LCMV, was propagated in BHK21 cells (33) . CFSE labeling. Leukocytes from donor mice were harvested and isolated as described previously (1) . The donor leukocytes were labeled with CFSE using a modification of previously described techniques (35, 36) . In brief, the splenocytes were suspended in HBBS (GIBCO BRL) at 2.5 ϫ 10 7 cells/ml and incubated in 2 M CFSE (Molecular Probes) solution for 15 min at 37ЊC. After incubation, donor cells were washed twice with HBSS.
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