Selected article for: "Cell viability and viable cell"
Author: Park, Jeong-In; Song, Kyung-Hee; Jung, Seung-Youn; Ahn, Jiyeon; Hwang, Sang-Gu; Kim, Joon; Kim, Eun Ho; Song, Jie-Young
Title: Tumor-Treating Fields Induce RAW264.7 Macrophage Activation Via NK-?B/MAPK Signaling Pathways
  • Document date: 2019_8_11
    • ID: 65s65ojc_11
    Snippet: Cell viability was determined by a trypan blue exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Amresco Life Science, Philadeplhia, Pennsylvania) assay. An equal volume of trypan blue reagent was added to the cell suspension, and the percentage of viable cells was evaluated by microscopy. 4T1 cells were plated in 24-well plates (1 Â 10 4 cells/well) for various times after treatment of the RAW 264.7 cell CM......
    Document: Cell viability was determined by a trypan blue exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Amresco Life Science, Philadeplhia, Pennsylvania) assay. An equal volume of trypan blue reagent was added to the cell suspension, and the percentage of viable cells was evaluated by microscopy. 4T1 cells were plated in 24-well plates (1 Â 10 4 cells/well) for various times after treatment of the RAW 264.7 cell CM. Then, MTT (5 mg/mL) reagent was added to each well for 3 hours, and absorbance was measured at 540 nm using a microplate reader (Multiskan EX, Thermo LabSystems, Waltham, Massachusetts). Assays were performed in triplicate.

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