Selected article for: "positive control and room temperature"
Author: Park, Jeong-In; Song, Kyung-Hee; Jung, Seung-Youn; Ahn, Jiyeon; Hwang, Sang-Gu; Kim, Joon; Kim, Eun Ho; Song, Jie-Young
Title: Tumor-Treating Fields Induce RAW264.7 Macrophage Activation Via NK-?B/MAPK Signaling Pathways
  • Document date: 2019_8_11
    • ID: 65s65ojc_15
    Snippet: RAW 264.7 cells at a density of 1 Â 10 5 cells/well were treated with TTFs or 1 ng/mL of LPS from Escherichia coli 055:B5 (L2880; Merck KGaA, Darmstadt, Germany) as a positive control for the activation of macrophages. After incubation, the culture supernatants of RAW 264.7 cells were collected, and each culture supernatant was mixed with the same amount of Griess reagent and incubated at room temperature for 10 minutes. The absorbance of the mi.....
    Document: RAW 264.7 cells at a density of 1 Â 10 5 cells/well were treated with TTFs or 1 ng/mL of LPS from Escherichia coli 055:B5 (L2880; Merck KGaA, Darmstadt, Germany) as a positive control for the activation of macrophages. After incubation, the culture supernatants of RAW 264.7 cells were collected, and each culture supernatant was mixed with the same amount of Griess reagent and incubated at room temperature for 10 minutes. The absorbance of the mixture was determined at 540 nm using a microplate reader (Multiskan EX, Thermo Fisher Scientific, Waltham, Massachusetts). All measurements were performed in triplicate. The amounts of nitrites were determined using a standard curve established with NaNO2.

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