Author: Saul, Vera Vivian; Seibert, Markus; Krüger, Marcus; Jeratsch, Sylvia; Kracht, Michael; Schmitz, Michael Lienhard
Title: ULK1/2 Restricts the Formation of Inducible SINT-Speckles, Membraneless Organelles Controlling the Threshold of TBK1 Activation Document date: 2019_8_6
ID: 5xk3z4ck_68
Snippet: GFP-Trap_A, Chromotek). Agarose beads were then washed four times with 1 ml cold IGEPAL buffer. Precipitated proteins were eluted by adding 1.5 × SDS sample buffer. Equal amounts of protein were separated by SDS-PAGE, followed by semidry blotting to a PVDF membrane (IPVH00010, Millipore). Further analysis was performed using standard methods......
Document: GFP-Trap_A, Chromotek). Agarose beads were then washed four times with 1 ml cold IGEPAL buffer. Precipitated proteins were eluted by adding 1.5 × SDS sample buffer. Equal amounts of protein were separated by SDS-PAGE, followed by semidry blotting to a PVDF membrane (IPVH00010, Millipore). Further analysis was performed using standard methods.
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