Author: Zakeri, Hamideh; Shokohi, Tahereh; Badali, Hamid; Mayahi, Saba; Didehdar, Mojtaba
Title: Use of Padlock Probes and Rolling Circle Amplification (RCA) for Rapid Identification of Trichophyton Species, Related to Human and Animal Disorder Document date: 2015_7_27
ID: 0y3pvht4_16
Snippet: Purified PCR products (1 µL) were mixed with 2 U Pfu (1 µL) DNA ligase (Bioneer ISO 13485, Alameda, CA, USA) and 1 µL of padlock probe in 500 mM Tris-HCl (pH 7.5), 50 mM KCl, 100 mM MgCl2, 2.5 µL/mL bovine serum albumin (pH 7.5), 10 mM ATP, and 50 mM DTT with a total reaction volume of 10 μL. Multiple cycle ligations were conducted with one cycle of denaturation at 94°C of 5 min, followed by five cycles at 94°C for 30 s and 4 min of ligati.....
Document: Purified PCR products (1 µL) were mixed with 2 U Pfu (1 µL) DNA ligase (Bioneer ISO 13485, Alameda, CA, USA) and 1 µL of padlock probe in 500 mM Tris-HCl (pH 7.5), 50 mM KCl, 100 mM MgCl2, 2.5 µL/mL bovine serum albumin (pH 7.5), 10 mM ATP, and 50 mM DTT with a total reaction volume of 10 μL. Multiple cycle ligations were conducted with one cycle of denaturation at 94°C of 5 min, followed by five cycles at 94°C for 30 s and 4 min of ligation at 62°C.
Search related documents:
Co phrase search for related documents- dna ligase and reaction volume: 1
- mm DTT and mm KCl: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- mm DTT and reaction volume: 1, 2, 3, 4, 5, 6
- mm KCl and reaction volume: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date