Author: Zakeri, Hamideh; Shokohi, Tahereh; Badali, Hamid; Mayahi, Saba; Didehdar, Mojtaba
Title: Use of Padlock Probes and Rolling Circle Amplification (RCA) for Rapid Identification of Trichophyton Species, Related to Human and Animal Disorder Document date: 2015_7_27
ID: 0y3pvht4_18
Snippet: RCA reactions were performed in a 25-μL volume containing 8 U of Bst DNA Polymerase (New England BioLabs, Ipswich, MA, USA), 200 µM deoxynucleoside triphosphate mix, 1 µL of each RCA primer, and 2 µL of ligation product. Probe signals were amplified by incubation at 65°C for 60 min and 85°C for 2 min, and the accumulation of doublestranded DNA products was detected by electrophoresis on 1% agarose containing ethidium bromide (Sigma, St. Lou.....
Document: RCA reactions were performed in a 25-μL volume containing 8 U of Bst DNA Polymerase (New England BioLabs, Ipswich, MA, USA), 200 µM deoxynucleoside triphosphate mix, 1 µL of each RCA primer, and 2 µL of ligation product. Probe signals were amplified by incubation at 65°C for 60 min and 85°C for 2 min, and the accumulation of doublestranded DNA products was detected by electrophoresis on 1% agarose containing ethidium bromide (Sigma, St. Louis, MO, USA). Ladder-like patterns were interpreted as positive reactions, while negative reactions showed no illumination.
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