Selected article for: "length genome and NGS sequencing"

Author: Folarin, Onikepe A.; Ehichioya, Deborah; Schaffner, Stephen F.; Winnicki, Sarah M.; Wohl, Shirlee; Eromon, Philomena; West, Kendra L.; Gladden-Young, Adrianne; Oyejide, Nicholas E.; Matranga, Christian B.; Deme, Awa Bineta; James, Ayorinde; Tomkins-Tinch, Christopher; Onyewurunwa, Kenneth; Ladner, Jason T.; Palacios, Gustavo; Nosamiefan, Iguosadolo; Andersen, Kristian G.; Omilabu, Sunday; Park, Daniel J.; Yozwiak, Nathan L.; Nasidi, Abdusallam; Garry, Robert F.; Tomori, Oyewale; Sabeti, Pardis C.; Happi, Christian T.
Title: Ebola Virus Epidemiology and Evolution in Nigeria
  • Document date: 2016_10_15
  • ID: 2g9ggwog_22
    Snippet: Raw sequencing reads from all sequencing runs were processed together and assembled using the viral-ngs pipeline (version 1.0.0) [12, 13] with mostly default parameters. Reads from 2 flow cells were not included owing to suspected contamination. Two parameters were varied from defaults: the minimum length of assembly (expressed as a fraction of the reference genome length) and minimum fraction of unambiguous bases were both decreased to allow ass.....
    Document: Raw sequencing reads from all sequencing runs were processed together and assembled using the viral-ngs pipeline (version 1.0.0) [12, 13] with mostly default parameters. Reads from 2 flow cells were not included owing to suspected contamination. Two parameters were varied from defaults: the minimum length of assembly (expressed as a fraction of the reference genome length) and minimum fraction of unambiguous bases were both decreased to allow assembly of lower-quality samples; these parameters were 0.8 and 0.7, respectively.

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