EndoU is a novel regulator of AICD during peripheral B cell selection

Poe, Jonathan C.; Kountikov, Evgueni I.; Lykken, Jacquelyn M.; Natarajan, Abirami; Marchuk, Douglas A.; Tedder, Thomas F.

littermates. Blast development after BCR ligation was assessed for purified spleen B cells from CD22 /[N1] littermates, along with B cells from single parental CD22 /[B6] and CD22 /[inbr] mice as controls. The results of 10 independent experiments were pooled. To reduce variability between mice and experiments, CD22 /[N1] mice B cell blast frequencies >1 SD above the mean percentage blasts were considered viable, whereas B cell blast frequencies <1 SD below the mean were considered nonviable. Genomic DNA from 22 viable and 22 nonviable CD22 /[N1] mice was used for genome-wide genotyping of 250 relevant SNPs between B6 and 129 mice (Illumina BeadArray; Duke University Genotyping Facility). QTL mapping used Map-Manager QTX regression analysis software (version QTXb20; Manly et al., 2001) . Informative SNPs between B6 and 129 mice were identified using the Mouse Genome Informatics and dbSNP databases. DNA primer pairs were used to PCR amplify these SNPs and their surrounding sequences, which were sequenced (Duke Cancer Center DNA Analysis Facility) to identify single nucleotide (homozygous B6:B6) or double nucleotide (heterozygous B6:129) peaks at SNP sites.