Selected article for: "gene expression and real time"
Author: Poe, Jonathan C.; Kountikov, Evgueni I.; Lykken, Jacquelyn M.; Natarajan, Abirami; Marchuk, Douglas A.; Tedder, Thomas F.
Title: EndoU is a novel regulator of AICD during peripheral B cell selection
  • Document date: 2014_1_13
    • ID: 5804sjmo_49
    Snippet: Gene expression analysis. Spleen B cells from mice of the indicated genotypes were cultured in triplicate for 18 h in medium alone or with F(ab) 2 anti-mouse IgM Ab, and then total RNA was isolated from pooled cells (RNeasy Plus; QIAGEN). For analysis of c-Myc and other signaling molecules, [ 32 P]-radiolabeled cDNA was generated and hybridized to GEArray NF-B signaling pathway gene arrays (SABiosciences) according to the manufacturer's ins.....
    Document: Gene expression analysis. Spleen B cells from mice of the indicated genotypes were cultured in triplicate for 18 h in medium alone or with F(ab) 2 anti-mouse IgM Ab, and then total RNA was isolated from pooled cells (RNeasy Plus; QIAGEN). For analysis of c-Myc and other signaling molecules, [ 32 P]-radiolabeled cDNA was generated and hybridized to GEArray NF-B signaling pathway gene arrays (SABiosciences) according to the manufacturer's instructions, with hybridization assessed using x-ray film. Gene expression within the Chr 15 locus was analyzed using Mouse 430.2 RNA arrays (Affymetrix; Duke Cancer Inst. DNA Analysis Facility) with GeneSpring GX software (Agilent Technologies) analysis. For real-time PCR analysis, cDNA was synthesized using random primers and analyzed using a LightCycler Instrument (software version 3) and FastStart DNA Master PLUS SYBR Green I kit (Roche Diagnostics Corp), with EndoU expression quantified using EndoU exon 5 (forward) 5-CGTCAACGAGAAGCTGTTCTCCAAG-3 and EndoU exon 6 (reverse) 5-CCACATGTTCTTCAAATCGTCCAC-3 primers. Cd20 expression was the internal control. Relative EndoU expression was quantified using the REST program (version 2).

    Search related documents:
    Co phrase search for related documents
    • Agilent Technologies analysis and Gene expression: 1
    • cDNA real time PCR analysis and Gene expression: 1
    • cDNA real time PCR analysis and Gene expression analysis: 1
    • cDNA real time PCR analysis and PCR analysis: 1
    • EndoU expression and Gene expression: 1
    • Gene expression analysis and internal control: 1, 2
    • Gene expression and GX software: 1
    • Gene expression and internal control: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • GeneSpring GX software and GX software: 1, 2