Selected article for: "cytometry analysis and flow cytometry"
Author: Jung, Juwon; Baek, Jin Ah; Seol, Hye Won; Choi, Young Min
Title: Propagation of Human Embryonic Stem Cells on Human Amniotic Fluid Cells as Feeder Cells in Xeno-Free Culture Conditions
  • Document date: 2016_3_23
    • ID: 20xe4vm6_14
    Snippet: After hESCs culture using hAFCs as feeder cells without animal derived components, a flow cytometer was used to determine whether Neu5Gc (Biolegend), an animal derived component, could be detected. Samples were prepared as described for the flow cytometry analysis of markers for undifferentiated cells after incubation with primary antibodies targeting Neu5Gc, followed by incubation with Cy5conjugated anti-chicken (Millipore) secondary antibodies......
    Document: After hESCs culture using hAFCs as feeder cells without animal derived components, a flow cytometer was used to determine whether Neu5Gc (Biolegend), an animal derived component, could be detected. Samples were prepared as described for the flow cytometry analysis of markers for undifferentiated cells after incubation with primary antibodies targeting Neu5Gc, followed by incubation with Cy5conjugated anti-chicken (Millipore) secondary antibodies.

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