Author: Mok, Hoyin; Cheng, Xing; Xu, Qi; Zengel, James R; Parhy, Bandita; Zhao, Jackie; Wang, C. Kathy; Jin, Hong
Title: Evaluation of Measles Vaccine Virus as a Vector to Deliver Respiratory Syncytial Virus Fusion Protein or Epstein-Barr Virus Glycoprotein gp350 Document date: 2012_2_16
ID: 3qdjmb2j_11
Snippet: Evaluation of recombinant measles viruses for their replication in cell cultures. Replication kinetics of rEZ, gp350, gp350tr, sF1 or sF3 viruses was compared in Vero cells. Vero cells in six-well plates were infected with each virus at a MOI of 0.01 in triplicates. After a 1 hour adsorption at room temperature, the cells were washed three times with phosphatebuffered saline (PBS) and incubated with 2 mL of complete DMEM at 35 o C with 5% CO 2 . .....
Document: Evaluation of recombinant measles viruses for their replication in cell cultures. Replication kinetics of rEZ, gp350, gp350tr, sF1 or sF3 viruses was compared in Vero cells. Vero cells in six-well plates were infected with each virus at a MOI of 0.01 in triplicates. After a 1 hour adsorption at room temperature, the cells were washed three times with phosphatebuffered saline (PBS) and incubated with 2 mL of complete DMEM at 35 o C with 5% CO 2 . Aliquots of culture supernatant were collected daily from each well for 7 days and stored at -80 o C until virus titration. Viral titers were determined by plaque assay in Vero cells as previously described [32] . Replication kinetic was performed twice in triplicate and each supernatant sample was titered in duplicate.
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