Author: Tromas, Nicolas; Zwart, Mark P.; Forment, Javier; Elena, Santiago F.
Title: Shrinkage of Genome Size in a Plant RNA Virus upon Transfer of an Essential Viral Gene into the Host Genome Document date: 2014_2_20
ID: 5fejitls_19
Snippet: Each mutation was introduced in the pMTEV infectious clone (Bedoya and Darò s 2010) by site-directed mutagenesis using the Quickchange II XL kit (Stratagene) and following the indications given by the manufacturer. To minimize unwanted errors during the mutagenesis process, the kit incorporates Pfu Ultra High-Fidelity Turbo DNA polymerase (Stratagene). The amplification conditions were 1 min at 95 C (initial denaturation), followed by 18 cycles .....
Document: Each mutation was introduced in the pMTEV infectious clone (Bedoya and Darò s 2010) by site-directed mutagenesis using the Quickchange II XL kit (Stratagene) and following the indications given by the manufacturer. To minimize unwanted errors during the mutagenesis process, the kit incorporates Pfu Ultra High-Fidelity Turbo DNA polymerase (Stratagene). The amplification conditions were 1 min at 95 C (initial denaturation), followed by 18 cycles consisting of 30 s at 95 C, 45 s at 65 C and 18 min at 68 C, and a final extension step of 28 min at 68 C.
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