Title: Signal recognition particle-dependent membrane insertion of mouse invariant chain: a membrane-spanning protein with a cytoplasmically exposed amino terminus Document date: 1986_6_1
ID: 4sw25blb_10
Snippet: Cell Free Protein Synthesis. Total or size fractionated mRNA was translated in a wheat germ cell free system (36) . The system was supplemented with either 2 A2~o U/ml of dog pancreas microsomes, pretreated with micrococcal nuclease and high salt, and/or 0.1 A2ao/ml of gradient-fractionated SRP (48) . Microsprees were prepared and treated as described previously (16, 32) . SRP was prepared essentially as described (48) with the exception that Nik.....
Document: Cell Free Protein Synthesis. Total or size fractionated mRNA was translated in a wheat germ cell free system (36) . The system was supplemented with either 2 A2~o U/ml of dog pancreas microsomes, pretreated with micrococcal nuclease and high salt, and/or 0.1 A2ao/ml of gradient-fractionated SRP (48) . Microsprees were prepared and treated as described previously (16, 32) . SRP was prepared essentially as described (48) with the exception that Nikkol was omitted in the fractionation on DEAE Sepharose and in the sucrose gradients. Except when otherwise stated, cell free translation was done at 25"C. Proteins were analyzed by SDS PAGE (28) and bands were visualized by fiuorography (7) using ENHANCE.
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