Selected article for: "essential medium and MEM essential medium"
Author: Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas; Hacker, Jill; Armstrong, R. Todd; White, Judith M.
Title: Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity
  • Document date: 1998_6_15
    • ID: 78fjem8s_10
    Snippet: Cells expressing wt-and mutant HAs were metabolically labeled with 35 S-TransLabel (ICN Pharmaceuticals, Inc., Costa Mesa, CA) as described previously (Kemble et al., 1993) . 24 h after transfection, cells were incubated with cys Ϫ /met Ϫ Minimal Essential medium (MEM; GIBCO BRL) for 45-90 min at 37 Њ C. The medium on each 10-cm plate was then replaced with 5 ml cys Ϫ /met Ϫ MEM containing 200 Ci 35 S-TransLabel and 2% supplemented calf seru.....
    Document: Cells expressing wt-and mutant HAs were metabolically labeled with 35 S-TransLabel (ICN Pharmaceuticals, Inc., Costa Mesa, CA) as described previously (Kemble et al., 1993) . 24 h after transfection, cells were incubated with cys Ϫ /met Ϫ Minimal Essential medium (MEM; GIBCO BRL) for 45-90 min at 37 Њ C. The medium on each 10-cm plate was then replaced with 5 ml cys Ϫ /met Ϫ MEM containing 200 Ci 35 S-TransLabel and 2% supplemented calf serum, and the cells were incubated in a CO 2 incubator at 37 Њ C for 14-18 h.

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