Author: Won, Hokeun; Lee, Dong-Uk; Jang, Guehwan; Noh, Yun-Hee; Lee, Seung-Chul; Choi, Hwan-Won; Yoon, In-Joong; Yoo, Han Sang; Lee, Changhee
Title: Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus Document date: 2019_7_9
ID: 2hxlx1j2_22
Snippet: Viral RNA was extracted from fecal suspensions prepared as described above using an i-TGE/ PED Detection Kit according to the manufacturer's protocol. Quantitative real-time RT-PCR was performed using a One Step SYBR PrimeScript RT-PCR Kit (TaKaRa, Japan) as described elsewhere [24, 30, 31] . A PEDV isolate with a known infectivity titer was 10-fold serially diluted to generate a standard curve in each PCR plate. The virus concentrations (TCID 50.....
Document: Viral RNA was extracted from fecal suspensions prepared as described above using an i-TGE/ PED Detection Kit according to the manufacturer's protocol. Quantitative real-time RT-PCR was performed using a One Step SYBR PrimeScript RT-PCR Kit (TaKaRa, Japan) as described elsewhere [24, 30, 31] . A PEDV isolate with a known infectivity titer was 10-fold serially diluted to generate a standard curve in each PCR plate. The virus concentrations (TCID 50 /mL) in samples were calculated based on the standard curve. The mean Ct values were calculated based on PCR positive samples, and the mean virus titers were calculated based on all pigs within the group.
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