Author: Won, Hokeun; Lee, Dong-Uk; Jang, Guehwan; Noh, Yun-Hee; Lee, Seung-Chul; Choi, Hwan-Won; Yoon, In-Joong; Yoo, Han Sang; Lee, Changhee
Title: Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus Document date: 2019_7_9
ID: 2hxlx1j2_6
Snippet: Vero cells (ATCC CCL-81) were cultured in alpha minimum essential medium (α-MEM; Invitrogen, USA) with 5% fetal bovine serum (FBS; Invitrogen) and antibiotic-antimycotic solutions (100×; Invitrogen) and maintained at 37°C in a humidified 5% CO 2 incubator. The isolation of a highly virulent Korean PEDV G2b strain, KOR/Aram/2014, was conducted from clinical fecal samples on Vero cells in the presence of trypsin (USB, USA). Virus isolation was c.....
Document: Vero cells (ATCC CCL-81) were cultured in alpha minimum essential medium (α-MEM; Invitrogen, USA) with 5% fetal bovine serum (FBS; Invitrogen) and antibiotic-antimycotic solutions (100×; Invitrogen) and maintained at 37°C in a humidified 5% CO 2 incubator. The isolation of a highly virulent Korean PEDV G2b strain, KOR/Aram/2014, was conducted from clinical fecal samples on Vero cells in the presence of trypsin (USB, USA). Virus isolation was confirmed by cytopathic effects (CPEs) observation, immunofluorescence assay (IFA), and nucleotide sequencing as described previously [23] . A viral stock was prepared from the 5th passage in cell culture (Aram-P5) and used as the parental and challenge virus in this study. PEDV N protein-specific monoclonal antibody (MAb) was obtained from ChoongAng Vaccine Laboratories (CAVAC; Korea).
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