Selected article for: "RNA synthesis and translation RNA synthesis"
Author: Sztuba-Solinska, Joanna; Teramoto, Tadahisa; Rausch, Jason W.; Shapiro, Bruce A.; Padmanabhan, Radhakrishnan; Le Grice, Stuart F. J.
Title: Structural complexity of Dengue virus untranslated regions: cis-acting RNA motifs and pseudoknot interactions modulating functionality of the viral genome
  • Document date: 2013_3_26
    • ID: 1pbd4maf_28
    Snippet: Previously, Manzano et al. (8) suggested putative roles of the TL/PK interactions in DENV replication. To disrupt potential TL1/PK2 and TL2/PK1 base pairing, they introduced point mutations by 'flipping' the corresponding sequences (TLs 5 0 -GCUGU-3 0 !5 0 -UGUCG-3 0 and PKs 5 0 -GCAGC-3 0 !5 0 -CGACG-3) in the context of the DENV2 replicon expressing Rluc. This strategy showed that the PK2 Flip mutation attenuated DENV Rluc replication, but not .....
    Document: Previously, Manzano et al. (8) suggested putative roles of the TL/PK interactions in DENV replication. To disrupt potential TL1/PK2 and TL2/PK1 base pairing, they introduced point mutations by 'flipping' the corresponding sequences (TLs 5 0 -GCUGU-3 0 !5 0 -UGUCG-3 0 and PKs 5 0 -GCAGC-3 0 !5 0 -CGACG-3) in the context of the DENV2 replicon expressing Rluc. This strategy showed that the PK2 Flip mutation attenuated DENV Rluc replication, but not translation, suggesting that PK2 region is crucial for viral RNA synthesis. As the DENV2 Rluc replicon encompassed the same cis-acting regulatory sequences as the DENV-MINI RNA used in our studies, we next sought to address the structure-function relation of PK2 mutations in the context of DENV-MINI RNA.

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