Selected article for: "cdna clone and infectious cdna clone"
Author: Mateo, Roberto; Nagamine, Claude M.; Kirkegaard, Karla
Title: Suppression of Drug Resistance in Dengue Virus
  • Document date: 2015_12_15
    • ID: 6bx2nrui_30
    Snippet: Dengue virus 2 serotype 16681 was propagated from infectious cDNA clone pD2IC/30P-A, a gift from Eva Harris, University of California, Berkeley, and originally developed by Kinney et al. 1997 (29) . Dengue virus serotype PL046 (17) was also generated from an infectious cDNA (a gift from Sujan Shresta, La Jolla Institute for Allergy and Immunology). All viruses were grown in C6/36 cells, and titers were determined in BHK-21 cells. For mouse experi.....
    Document: Dengue virus 2 serotype 16681 was propagated from infectious cDNA clone pD2IC/30P-A, a gift from Eva Harris, University of California, Berkeley, and originally developed by Kinney et al. 1997 (29) . Dengue virus serotype PL046 (17) was also generated from an infectious cDNA (a gift from Sujan Shresta, La Jolla Institute for Allergy and Immunology). All viruses were grown in C6/36 cells, and titers were determined in BHK-21 cells. For mouse experiments, virus was concentrated by ultracentrifugation at 53,000 ϫ g for 2 h at 4°C and resuspended in cold endotoxin-free phosphate-buffered saline supplemented with 10% fetal bovine serum.

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