Title: The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal Document date: 1993_3_1
ID: 377v2ufn_11
Snippet: Plasmid AG-LMBR was constructed to express a chimeric protein in which the first 191 amino acids of LBR were replaced by chimpanzee a-globin. ~globin eDNA was amplified by PCR as described in the preceding paragraph but with a different antisense primer that did not extend to the TAA stop codon and had a BspE1 site added in frame 5' to the last three nucleotides of the coding sequence. The PCR product was purified from the reaction mixture by phe.....
Document: Plasmid AG-LMBR was constructed to express a chimeric protein in which the first 191 amino acids of LBR were replaced by chimpanzee a-globin. ~globin eDNA was amplified by PCR as described in the preceding paragraph but with a different antisense primer that did not extend to the TAA stop codon and had a BspE1 site added in frame 5' to the last three nucleotides of the coding sequence. The PCR product was purified from the reaction mixture by phenol:chloroform extraction, digested with EcoRI and BspE1, purified again and ligated into EcoRI/BspE1 digested plasmid LMBR.
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