The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal

The eDNA sequence of chicken LBR and descriptions of LBR clones have been previously published . To construct plasmid LMBR that codes for full-length chicken LBR, a PCR product from nucleotide +1210 to nucleotide +1914 was amplified using as template a mixture of DNA from previously described clones M-3 and M-7. A HindIII site was engineered at the 5' end of the antisense primer. The product was purified from the reaction mixture using the GeneClean II Kit (Bin 101, Inc., Vista, CA), cut with NheI (NheI site at nucleotide +1303 of LBR eDNA) and HindlII, purified again and ligated into LBR clone DJ-5 that was cut with NheI and HindlII. The resulting recombinant clone, which contained cDNA coding for full-length LBR, was digested with EcoRI and SalI and the cDNA insert isolated from agarose gels and ligated into the EcoRI/SalI site of pSVK3.