Title: The first membrane spanning region of the lamin B receptor is sufficient for sorting to the inner nuclear membrane Document date: 1993_2_1
ID: 3qi2llmr_14
Snippet: For immunoprecipitation, the in vitro translation reactions were diluted fivefold with dilution buffer (50 mM "his, pH 8.0, 1% NP-40, 150 mM NaC1, I mM PMSF) and incubated with 5/~1 of control guinea pig serum or anti-p58 guinea pig serum (Worman et al., 1988) . The immune complexes were precipitated with protein A-Sepharose (Pharmacia Fine Chemicais), washed three times with dilution buffer, and then analyzed by SDS-PAGE as described above......
Document: For immunoprecipitation, the in vitro translation reactions were diluted fivefold with dilution buffer (50 mM "his, pH 8.0, 1% NP-40, 150 mM NaC1, I mM PMSF) and incubated with 5/~1 of control guinea pig serum or anti-p58 guinea pig serum (Worman et al., 1988) . The immune complexes were precipitated with protein A-Sepharose (Pharmacia Fine Chemicais), washed three times with dilution buffer, and then analyzed by SDS-PAGE as described above.
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