Title: The v-sis oncoprotein loses transforming activity when targeted to the early Golgi complex Document date: 1994_12_2
ID: 2otgb2w8_25
Snippet: proteins constructed for these experiments. The first three constructs, sis-G, sis-G-ER +, and sisG-ER-, were described previously , and served as a basic model for the construction of these new fusion proteins. In all cases, the first 239 amino acids of v-sis are included in the fusion proteins. This portion of v-sis provides a signal sequence, a dibasic proteolytic processing site, and the 82-amino acid minimal transforming region (Hannink and .....
Document: proteins constructed for these experiments. The first three constructs, sis-G, sis-G-ER +, and sisG-ER-, were described previously , and served as a basic model for the construction of these new fusion proteins. In all cases, the first 239 amino acids of v-sis are included in the fusion proteins. This portion of v-sis provides a signal sequence, a dibasic proteolytic processing site, and the 82-amino acid minimal transforming region (Hannink and Donoghue, 1986b; Sauer et al., 1986; Giese et al., 1987; Sauer and Donoghue, 1988) . All constructs are in Type I orientation-the NH2 terminus is "out; and the COOH terminus "in"
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