Selected article for: "laser desorption and mass spectrometry"
Author: Wang, Yuan; Li, Yan; Ding, Tianbing
Title: Heat shock protein 90ß in the Vero cell membrane binds Japanese encephalitis virus
  • Document date: 2017_6_26
    • ID: 7cpxg1b4_11
    Snippet: Mass spectrometry (MS) analysis. Novel stained bands of the Co-IP immunocomplex on SDS-PAGE gels were excised for matrix-assisted laser desorption ionization-time of flight MS (MALDI-TOF MS). Briefly, a gel with a single band was minced into sections at approximately 1 mm 3 and transferred into a sterile microcentrifuge tube. The gel sections were immersed in a 500 µl preservation solution (50% acetonitrile, 50% distilled water) and mailed to th.....
    Document: Mass spectrometry (MS) analysis. Novel stained bands of the Co-IP immunocomplex on SDS-PAGE gels were excised for matrix-assisted laser desorption ionization-time of flight MS (MALDI-TOF MS). Briefly, a gel with a single band was minced into sections at approximately 1 mm 3 and transferred into a sterile microcentrifuge tube. The gel sections were immersed in a 500 µl preservation solution (50% acetonitrile, 50% distilled water) and mailed to the laboratory in Beijing (see Acknowledgements) at room temperature for further MS processing protocol and analysis. Protein identity was determined based on a score of >30 from the homology search tool MASCOT, where the mass/charge values matched the information available in primate databases (28) .

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