Use of Padlock Probes and Rolling Circle Amplification (RCA) for Rapid Identification of Trichophyton Species, Related to Human and Animal Disorder

Zakeri, Hamideh; Shokohi, Tahereh; Badali, Hamid; Mayahi, Saba; Didehdar, Mojtaba

The approximate length of the three specific circular oligonucleotide probes for T. rubrum, T. mentagrophytes var. interdigitale, and T. tonsurans used in this study was 96 to 102 bp, and comprised two target-complementary segments connected by a genetic linker sequence. They were previously designed by Kong et al. (8) to minimize similarity between closely related strains and to allow primer binding during RCA. However, specific padlock probes targeting the ITS1 and ITS2 regions were modified, and RCA primers designed to specifically bind the linker region of the probes were synthesized by Anaspec Inc. (San Jose, CA, USA) ( Table 1 ).