Selected article for: "DNA virus and envelope protein"
Author: Zhou, ShengTao; Liu, Rui; Zhao, Xia; Huang, CanHua; Wei, YuQuan
Title: Viral proteomics: The emerging cutting-edge of virus research
  • Document date: 2011_6_26
    • ID: 3ahamzjv_12
    Snippet: Other enveloped DNA viruses have been studied using an integrated proteomics strategy, for example, white spot syndrome virus (WSSV). WSSV is a double-stranded DNA virus that contains a 305-kb circular DNA, which putatively encodes 181 ORFs. In 2002, Huang et al. [18] separated the purified WSSV virions by SDS-PAGE, excised 24 protein bands, and subjected them to in-gel trypsin digestion, MALDI-TOF, and electrospray ionization tandem mass spectro.....
    Document: Other enveloped DNA viruses have been studied using an integrated proteomics strategy, for example, white spot syndrome virus (WSSV). WSSV is a double-stranded DNA virus that contains a 305-kb circular DNA, which putatively encodes 181 ORFs. In 2002, Huang et al. [18] separated the purified WSSV virions by SDS-PAGE, excised 24 protein bands, and subjected them to in-gel trypsin digestion, MALDI-TOF, and electrospray ionization tandem mass spectrometry. Eighteen proteins corresponding to ORFs of the WSSV genome were identified, among which were 14 proteins of unknown functions. Temporal analysis implied that all the genes were transcribed in the late stage of WSSV infection, except for vp121. Of the 14 newly-identified proteins, VP466 was further characterized as a novel envelope protein. More recent research described the application of the isobaric tags for relative and absolute quantification (iTRAQâ„¢) approach to define the location of proteins in WSSV [19] . Li et al. [19] reported 45 viral proteins, including 13 novel proteins associated with the virion. In particular, they identified 23 envelope proteins and six nucleocapsid proteins, including 12 novel envelopes and two novel nucleocapsid proteins. This study exemplifies the successful application of quantitation techniques to investigate the localization of virion proteins.

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