Impact of dietary spray-dried bovine plasma addition on pigs infected with porcine epidemic diarrhea virus

Duffy, Mark A; Chen, Qi; Zhang, Jianqiang; Halbur, Patrick G; Opriessnig, Tanja

The statistical software used was JMP Pro 11. Analysis of variance (ANOVA) was used for cross-sectional assessment of the average daily weight gain and continuous measures including viral shedding, IgA, and IgG antibody levels. The PEDV genomic copy numbers per milliliter of fecal suspension were log transformed before statistical analysis. If a significant (P < 0.05) difference was detected, pairwise testing using the Tukey-Kramer adjustment was performed to determine which groups were different. Daily rectal temperature data were analyzed with multivariate ANOVA. Non-repeated measures of necropsy and histopathology data were assessed using nonparametric Kruskal-Wallis ANOVA. If a nonparametric ANOVA test was significant (P < 0.05), then Wilcoxon tests were used to assess the differences between pairs of groups. Differences in incidence of clinical scores were evaluated by using the Fisher's exact test. For fecal shedding of PEDV RNA, an area under the curve (AUC) was calculated for each animal individually and differences between groups were assessed by unpaired t-test (data passed Shapiro-Wilks normality test).