Author: Swatek, Kirby N.; Aumayr, Martina; Pruneda, Jonathan N.; Visser, Linda J.; Berryman, Stephen; Kueck, Anja F.; Geurink, Paul P.; Ovaa, Huib; van Kuppeveld, Frank J. M.; Tuthill, Tobias J.; Skern, Tim; Komander, David
Title: Irreversible inactivation of ISG15 by a viral leader protease enables alternative infection detection strategies Document date: 2018_3_6
ID: 3s86w4iw_9
Snippet: This surprising result was corroborated in additional experiments. We compared the cleavage rates of pro-, mature-, and substrate-bound forms of ISG15 by intact MS analysis, revealing similar activity of Lb pro against pro-and mature ISG15 and slightly higher activity toward ISG15 linked to 5-tetramethylrhodamine (TAMRA)-labeled Lys-Gly dipeptide via an isopeptide bond (Fig. S1G ). This showed that Lb pro can process all forms of ISG15 present in.....
Document: This surprising result was corroborated in additional experiments. We compared the cleavage rates of pro-, mature-, and substrate-bound forms of ISG15 by intact MS analysis, revealing similar activity of Lb pro against pro-and mature ISG15 and slightly higher activity toward ISG15 linked to 5-tetramethylrhodamine (TAMRA)-labeled Lys-Gly dipeptide via an isopeptide bond (Fig. S1G ). This showed that Lb pro can process all forms of ISG15 present in cells.
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