Author: Strating, Jeroen R.P.M.; van der Linden, Lonneke; Albulescu, Lucian; Bigay, Joëlle; Arita, Minetaro; Delang, Leen; Leyssen, Pieter; van der Schaar, Hilde M.; Lanke, Kjerstin H.W.; Thibaut, Hendrik Jan; Ulferts, Rachel; Drin, Guillaume; Schlinck, Nina; Wubbolts, Richard W.; Sever, Navdar; Head, Sarah A.; Liu, Jun O.; Beachy, Philip A.; De Matteis, Maria A.; Shair, Matthew D.; Olkkonen, Vesa M.; Neyts, Johan; van Kuppeveld, Frank J.M.
Title: ITRACONAZOLE INHIBITS ENTEROVIRUS REPLICATION BY TARGETING THE OXYSTEROL-BINDING PROTEIN Document date: 2015_1_29
ID: 3kmqy07w_14
Snippet: HeLa R19 cells (2 x 10 3 cells/well of a 96-well plate) were reverse transfected with 2 pmol of small interfering RNA (siRNA) per well using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. Scramble siRNA (AllStars Negative Control; Qiagen) was used as a negative control, siRNA against PI4KIIIβ (5'-UGUUGGGGCUUCCCUGCCCTT-3') was from Qiagen, and siRNAs against OSBP (two siRNAs mixed at a 1:1 ratio, 2 pmol total per we.....
Document: HeLa R19 cells (2 x 10 3 cells/well of a 96-well plate) were reverse transfected with 2 pmol of small interfering RNA (siRNA) per well using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. Scramble siRNA (AllStars Negative Control; Qiagen) was used as a negative control, siRNA against PI4KIIIβ (5'-UGUUGGGGCUUCCCUGCCCTT-3') was from Qiagen, and siRNAs against OSBP (two siRNAs mixed at a 1:1 ratio, 2 pmol total per well, 5'-
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