Localization of the Lys, Asp, Glu, Leu tetrapeptide receptor to the Golgi complex and the intermediate compartment in mammalian cells

We next investigated the localization of the KDEL-R in two commonly used lines, HeLa (Fig. 2) and NRK (not shown). In both cell types a strong labeling was seen on and around the Golgi stack. In the majority of cells, the labeling was associated with one cisterna on one side of the Golgi stack, as well as more peripheral membrane elements, which we assume to be the IC (Fig. 2 A) . However, we also saw many examples where the Golgi stack was essentially free of labeling, while the peripheral elements labeled significantly (Fig. 2 B) . Conversely, a few Golgi complexes were seen which had labeling throughout the stack (not shown).