Author: Kim, Nam-Gun; Kim, Myeong-Ae; Park, Young-Il; Jung, Tae-Sung; Son, Seong-Wan; So, ByungJae; Kang, Hwan-Goo
Title: Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin Document date: 2015_12_17
ID: 5i84nomn_18
Snippet: Antigen coating of the microplate and blocking were carried out as described above. After removal of the blocking solution, standard ENR or sample ENR (80 ïL/well) were added to each well, followed by the addition of ENR antibody-HRP conjugate (80 ïL/well) and incubation at room temperature for 1 h. After washing three times, color development was conducted as described in the indirect competitive ELISA screening procedure......
Document: Antigen coating of the microplate and blocking were carried out as described above. After removal of the blocking solution, standard ENR or sample ENR (80 ïL/well) were added to each well, followed by the addition of ENR antibody-HRP conjugate (80 ïL/well) and incubation at room temperature for 1 h. After washing three times, color development was conducted as described in the indirect competitive ELISA screening procedure.
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