Title: Membrane insertion of gap junction connexins: polytopic channel forming membrane proteins Document date: 1994_10_2
ID: 1gqffey0_11
Snippet: Rat cq and/31 GJ proteins were expressed in BHK cells after cq and/~l cDNAs were stably transfected into these cells as described in Kumar and Gilula (1992) . GJ cDNAs were cloned into the BamHI-site of exon I of human growth hormone encoded in the eucaryotic expression vector pNUT, kindly provided by Dr. R. D. Palmiter (University of Washington, Seattle, WA). With this strategy, the GJ cDNAs are under the control of an inducible metallothionin p.....
Document: Rat cq and/31 GJ proteins were expressed in BHK cells after cq and/~l cDNAs were stably transfected into these cells as described in Kumar and Gilula (1992) . GJ cDNAs were cloned into the BamHI-site of exon I of human growth hormone encoded in the eucaryotic expression vector pNUT, kindly provided by Dr. R. D. Palmiter (University of Washington, Seattle, WA). With this strategy, the GJ cDNAs are under the control of an inducible metallothionin promoter that allows the expression of variable amounts of GJ protein. BHK cells were cotransfected by standard CaPOa cotransfection procedures, and recombinants were selected by methotrexate treatment.
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