Author: Menachery, Vineet D.; Schäfer, Alexandra; Burnum-Johnson, Kristin E.; Mitchell, Hugh D.; Eisfeld, Amie J.; Walters, Kevin B.; Nicora, Carrie D.; Purvine, Samuel O.; Casey, Cameron P.; Monroe, Matthew E.; Weitz, Karl K.; Stratton, Kelly G.; Webb-Robertson, Bobbie-Jo M.; Gralinski, Lisa E.; Metz, Thomas O.; Smith, Richard D.; Waters, Katrina M.; Sims, Amy C.; Kawaoka, Yoshihiro; Baric, Ralph S.
Title: MERS-CoV and H5N1 influenza virus antagonize antigen presentation by altering the epigenetic landscape Document date: 2018_1_30
ID: 096gtdy5_29
Snippet: Cells and Viruses. Calu3 cells were utilized as described (37, 38, 40) . Viral titrations and propagation of SARS-CoV, MERS-CoV, and influenza virus were performed in VeroE6, Vero-81, and Madin-Darby canine kidney (MDCK) cells by using standard methods. WT icSARS-CoV was derived from the R.S.B. laboratory's infectious clone constructs (41) . MERS-CoV (EMC 2012 strain) was provided by Bart L. Haagmans, Erasmus Medical Center, Rotterdam, The Nether.....
Document: Cells and Viruses. Calu3 cells were utilized as described (37, 38, 40) . Viral titrations and propagation of SARS-CoV, MERS-CoV, and influenza virus were performed in VeroE6, Vero-81, and Madin-Darby canine kidney (MDCK) cells by using standard methods. WT icSARS-CoV was derived from the R.S.B. laboratory's infectious clone constructs (41) . MERS-CoV (EMC 2012 strain) was provided by Bart L. Haagmans, Erasmus Medical Center, Rotterdam, The Netherlands via material transfer agreement. H5N1-VN1203 was derived from a plasmid-based reverse-genetic system, and H1N1-09 was from WT stock; both were subsequently amplified in MDCK cells (42) .
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