miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1

Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao

To obtain a comprehensive quantitative expression profile of miRNAs in mouse bone marrow-derived DCs at different differentiation stages, in this study we establish miRNomes in bone marrow HSCs, imDCs, maDCs (in vitro and in vivo) and DCreg (in vitro and in vivo) using next-generation sequencing on Illumina platform. We demonstrate that about 391 miRNAs are differentially expressed during DC differentiation, with numerous stage-specific miRNAs regulated by histone modifications at different stages. A total of 121 miRNAs have the same alteration pattern during DC differentiation process (maDCs-DCreg) in vitro and in vivo. miR-30b, significantly upregulated in DCreg both in vitro and in vivo, is found to promote IL-10 and NO production through targeting Notch1. In addition, we reveal 53 new miRNA candidates. Our results may present a resource for researching the roles of miRNAs in the development and differentiation of DCs and also stem cell biology.